MOESM3 of Weight loss reduces basal-like breast cancer through kinome reprogramming Yuanyuan Qin Sneha Sundaram Luma Essaid Xin Chen Samantha Miller Feng Yan David Darr Joseph Galanko Stephanie Montgomery Michael Major Gary Johnson Melissa Troester Liza Makowski 10.6084/m9.figshare.c.3630467_D1.v1 https://springernature.figshare.com/articles/figure/MOESM3_of_Weight_loss_reduces_basal-like_breast_cancer_through_kinome_reprogramming/4422197 Additional file 3. Kinome profiling revealed significant regulation of pathways by HFD that were reversed with weight loss. A and b. Quantitative comparison of kinases in unaffected mammary tissues from mice using MIB/MS was conducted. Legend indicates three iTRAQ runs with 2–4 samples pooled per group per run. The graphs indicates quantitative changes in kinase activity as a ratio of mice fed 60 % (a) or 60–10 % (b) diet relative to mice fed 10 % diet group. Ratio <1 denotes decreased kinase activity and >1 increased kinase activity. Kinase families are indicated (AGC: Containing PKA, PKG, PKC families; CAMK: Calcium/calmodulin-dependent protein kinase; CK1: Casein kinase 1; CMGC: Containing CDK, MAPK, GSK3, CLK families; STE: Homologs of yeast Sterile 7, Sterile 11, Sterile 20 kinases; TK: Tyrosine kinase; TKL: Tyrosine kinase-like). c. Mean kinase activity is reported for mice fed 60 % diet (dark grey) or 60–10 % diet (light grey) compared to mice on 10 % diet group. Error bars are not indicated for clarity. Statistically significant comparisons are reported in Fig. 5d. 2016-04-01 05:00:00 Kinome MAPK AMPK obesity Leptin High fat diet Adiposity Body composition