MOESM3 of Weight loss reduces basal-like breast cancer through kinome reprogramming
Yuanyuan Qin
Sneha Sundaram
Luma Essaid
Xin Chen
Samantha Miller
Feng Yan
David Darr
Joseph Galanko
Stephanie Montgomery
Michael Major
Gary Johnson
Melissa Troester
Liza Makowski
10.6084/m9.figshare.c.3630467_D1.v1
https://springernature.figshare.com/articles/figure/MOESM3_of_Weight_loss_reduces_basal-like_breast_cancer_through_kinome_reprogramming/4422197
Additional file 3. Kinome profiling revealed significant regulation of pathways by HFD that were reversed with weight loss. A and b. Quantitative comparison of kinases in unaffected mammary tissues from mice using MIB/MS was conducted. Legend indicates three iTRAQ runs with 2–4 samples pooled per group per run. The graphs indicates quantitative changes in kinase activity as a ratio of mice fed 60 % (a) or 60–10 % (b) diet relative to mice fed 10 % diet group. Ratio <1 denotes decreased kinase activity and >1 increased kinase activity. Kinase families are indicated (AGC: Containing PKA, PKG, PKC families; CAMK: Calcium/calmodulin-dependent protein kinase; CK1: Casein kinase 1; CMGC: Containing CDK, MAPK, GSK3, CLK families; STE: Homologs of yeast Sterile 7, Sterile 11, Sterile 20 kinases; TK: Tyrosine kinase; TKL: Tyrosine kinase-like). c. Mean kinase activity is reported for mice fed 60 % diet (dark grey) or 60–10 % diet (light grey) compared to mice on 10 % diet group. Error bars are not indicated for clarity. Statistically significant comparisons are reported in Fig. 5d.
2016-04-01 05:00:00
Kinome
MAPK
AMPK obesity
Leptin
High fat diet
Adiposity
Body composition