%0 Figure %A Navarro, Gemma %A Cordomí, Arnau %A Zelman-Femiak, Monika %A Brugarolas, Marc %A Moreno, Estefania %A Aguinaga, David %A Perez-Benito, Laura %A Cortés, Antoni %A Casadó, Vicent %A Mallol, Josefa %A Canela, Enric %A Lluís, Carme %A Pardo, Leonardo %A García-Sáez, Ana %A McCormick, Peter %A Franco, Rafael %D 2016 %T Additional file 5: Figure S5. of Quaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs %U https://springernature.figshare.com/articles/figure/Additional_file_5_Figure_S5_of_Quaternary_structure_of_a_G-protein-coupled_receptor_heterotetramer_in_complex_with_Gi_and_Gs/4419512 %R 10.6084/m9.figshare.c.3629618_D3.v1 %2 https://springernature.figshare.com/ndownloader/files/7146158 %K GPCR %K Heterotetramer %K Heterotrimeric G protein %K Single-particle tracking %K BRET %K Molecular modeling %X BRET assays in cells expressing fusion proteins containing hemi-Rluc8 and hemi-Venus moieties fused to adenosine receptors or containing the ghrelin GHS1a receptor instead of one of the adenosine receptors. (A) Saturation BRET curve in HEK-293T co-transfected with 1.5 μg of the two cDNAs corresponding to A1R-cRLuc8 and A2AR-nRLuc8 and with increasing amounts of cDNAs corresponding to A1R-nVenus and A2AR-cVenus (equal amounts of the two cDNAs). BRETmax was 35 ± 2 mBU and BRET50 was 16 ± 3 mBU. BRET in cells expressing cRluc8 instead of A1R-cRluc8 gave a linear, non-saturable signal. (B) Comparison of BRET responses using complementary and non-complementary pairs, or replacing one adenosine receptor with the ghrelin GHS1a (gn) receptor. Data are mean ± SD of three different experiments grouped as a function of the amount of BRET acceptor. ***p < 0.001 with respect to BRET in cells expressing adenosine receptors and hemi-Rluc8 and hemi-Venus proteins. (TIF 398 kb) %I figshare