Additional file 1: Figure S1. of Metabolic determinants of the immune modulatory function of neural stem cells Denise Drago Veronica Basso Edoardo Gaude Giulio Volpe Luca Peruzzotti-Jametti Angela Bachi Giovanna Musco Annapaola Andolfo Christian Frezza Anna Mondino Stefano Pluchino 10.6084/m9.figshare.c.3629558_D2.v1 https://springernature.figshare.com/articles/figure/Additional_file_1_Figure_S1_of_Metabolic_determinants_of_the_immune_modulatory_function_of_neural_stem_cells/4419290 NSCs induce downregulation of the activation cell surface marker CD44 on purified CD4+ T cells. CD4+ T cells were purified by negative selection from total LNC, labelled with the vital dye CFSE, and cultured on CD3/CD28 coated mAb in the absence or the presence of NSCs, Th1 NSCs or Th2 NSCs for 72 h. Cells were surface stained with anti-CD4 and CD44 antibodies. Absolute fractions of CFSEdimCD44low CD4+ T cells are depicted. Data are expressed as mean % (±SD) from n ≥ 3 independent experiments. *p ≤ 0.05 and **p ≤ 0.01 vs. T cells. 2016-09-02 05:00:00 Metabolomics Neural stem cells Immune modulation Lymph node cells Arginase I