10.6084/m9.figshare.c.3629282_D7.v1 Natalie Landeck Natalie Landeck Hélène Hall Hélène Hall Mustafa Ardah Mustafa Ardah Nour Majbour Nour Majbour Omar El-Agnaf Omar El-Agnaf Glenda Halliday Glenda Halliday Deniz Kirik Deniz Kirik Additional file 3: Figure S3. of A novel multiplex assay for simultaneous quantification of total and S129 phosphorylated human alpha-synuclein Springer Nature 2016 Alpha-synuclein AlphaLISA Multiplex assay Phosphorylated alpha-synuclein Synucleinopathy 2016-08-22 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_Figure_S3_of_A_novel_multiplex_assay_for_simultaneous_quantification_of_total_and_S129_phosphorylated_human_alpha-synuclein/4418540 Scheme of AlphaLISA duplex assay for total and S129 phosphorylated human alpha-synuclein detection. The 4B12 antibody – binding to aa 103–108 – is biotinylated and used to bind the Streptavidin-coated Donor-beads. Terbium Acceptor-beads were coupled to LB509 antibodies for total h-asyn quantification while the 11A5 antibody was used and attached to Europium Acceptor-beads in order to measure pS129 h-asyn. Both antibodies recognize an epitope close to each other (aa 115–122 and aa 129 respectively). (PDF 500 kb)