%0 Journal Article %A Jandu, Haatisha %A Aluzaite, Kristina %A Fogh, Louise %A Thrane, Sebastian %A Noer, Julie %A Proszek, Joanna %A Do, Khoa %A Hansen, Stine %A Damsgaard, Britt %A Nielsen, Signe %A Stougaard, Magnus %A Knudsen, Birgitta %A Moreira, José %A Hamerlik, Petra %A Gajjar, Madhavsai %A Smid, Marcel %A Martens, John %A Foekens, John %A Pommier, Yves %A Brünner, Nils %A Schrohl, Anne-Sofie %A Stenvang, Jan %D 2016 %T Additional file 1: of Molecular characterization of irinotecan (SN-38) resistant human breast cancer cell lines %U https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_of_Molecular_characterization_of_irinotecan_SN-38_resistant_human_breast_cancer_cell_lines/4413287 %R 10.6084/m9.figshare.c.3627530_D1.v1 %2 https://springernature.figshare.com/ndownloader/files/7139672 %K Breast cancer %K Topoisomerase I %K Irinotecan %K SN-38 %K Resistance %K ABCG2/BCRP %X This file contains the following: Table S1: a table with FISH data of TOP1/CEN-20 and TOP2A/CEN-17, TOP1/CEN-20 analyses of 52 human breast cancer cell lines. Figure S1: correlations between TOP1 copynumbers from FISH and SNP analyses, and correlation between TOP1 copy numbers and gene expression, presentation of 9 breast cancer cell lines with regards to TOP1 copy numbers, ER and HER2 status and the sensitivity to SN-38. Figure S2: consistency between Top1 protein levels and TOP1 copy numbers, Top1 enzymatic activity in 9 breast cancer cell lines and the influence of SN-38 on Top1 enzymatic activity, dose–response of 9 breast cancer cell lines to SN-38 and IC50 values. Figure S3: MTT data demonstration that the the acquired and de novo SN-38 resitant cell lines are resistant when grown in the concentration of SN-38 at which the the development of resistance was terminated. Figure S4: Growth curves of the SN-38 resistant cell lines in comparison to their parental and DMSO controls. Figure S5: Cell cycle distribution of resistant cell lines in comparison to their respective DMSO controls. Figure S6: Sensitivity to SN-38 in docetaxel resistant breast cancer cell lines. Figure S7: Global expression analysis showing sample clustering, the number of differentially expressed genes between resistant and wild type DMSO control cell lines and Enrichment of Gene Ontology Molecular Function. Figure S8: Western blotting of Top2a in SN-38 resistant cell lines in comparison to their respective controls. Figure S9: Nannocapillary analysis of Top1 and β-actin in SN-38 resistant MCF7 and MCF7 control cells. Figure S10: Western blotting of ID3 in SN-38 resistant MDA-MB-231 and MCF-7 cells and their controls cells. (PDF 757 kb) %I figshare