10.6084/m9.figshare.c.3627482_D2.v1
Akihisa Okamoto
Akihisa
Okamoto
Masahiro Tanaka
Masahiro
Tanaka
Chisato Sumi
Chisato
Sumi
Kanako Oku
Kanako
Oku
Munenori Kusunoki
Munenori
Kusunoki
Kenichiro Nishi
Kenichiro
Nishi
Yoshiyuki Matsuo
Yoshiyuki
Matsuo
Keizo Takenaga
Keizo
Takenaga
Koh Shingu
Koh
Shingu
Kiichi Hirota
Kiichi
Hirota
Additional file 1: Figure S1. of The antioxidant N-acetyl cysteine suppresses lidocaine-induced intracellular reactive oxygen species production and cell death in neuronal SH-SY5Y cells
Springer Nature
2016
Lidocaine
Mitochondria
ROS
Redox
Apoptosis
Necrosis
Oxygen consumption
2016-10-24 05:00:00
Journal contribution
https://springernature.figshare.com/articles/journal_contribution/Additional_file_1_Figure_S1_of_The_antioxidant_N-acetyl_cysteine_suppresses_lidocaine-induced_intracellular_reactive_oxygen_species_production_and_cell_death_in_neuronal_SH-SY5Y_cells/4413152
Analysis of cell apoptosis by FACS. Levels of cell apoptosis were measured using an Annexin V-FITC Apoptosis Detection Kit (BioVision, Milpitas, CA, USA), according to the manufacturer’s instructions. For these analyses, SH-SY5Y cells were seeded into 6-well plates (3 × 105 cells/well) and incubated overnight. The following day, cells were treated with the indicated concentrations of the appropriate drug(s) for varying lengths of time and harvested by centrifugation at 1200 rpm for 3 min. The culture supernatants were discharged, and the resulting pellets were resuspended in a mixture comprised of 500 μl binding buffer, 5 μl Annexing V-FITC, and 5 μl propidium iodide (PI; 50 μg/ml) for 5 min at room temperature in the dark and analyzed using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA). (PDF 2009 kb)