10.6084/m9.figshare.c.3622250_D5.v1
Kyeong-Su Park
Kyeong-Su
Park
Ju Kim
Ju
Kim
Hee Shin
Hee
Shin
Kyung-Sook Chung
Kyung-Sook
Chung
Dong-Soo Im
Dong-Soo
Im
Jung Lim
Jung
Lim
Cho-Rok Jung
Cho-Rok
Jung
Additional file 2: Figure S2. of E2-EPF UCP regulates stability and functions of missense mutant pVHL via ubiquitin mediated proteolysis
Springer Nature
2015
VHL disease
pVHL missense mutation
E2-EPF UCP
Ubiquitination
Protein instability
2015-10-26 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_E2-EPF_UCP_regulates_stability_and_functions_of_missense_mutant_pVHL_via_ubiquitin_mediated_proteolysis/4397393
Missense mutant pVHL constitute E3 ubiquitin complex in cell. (A) HEK293T cells were transfected with plasmid expressing GST tagged hotspot mutant pVHL and/or Flag tagged HIF-1Îą protein and then detected the interaction using GST-pull down assay. (B) HEK293T cells were transfected with plasmid expressing HA tagged mutant pVHL and/or GST tagged Elongin C protein and then detected the interaction using GST-pull down assay. Interacted mutants VHL proteins were detected by immunoblot as indicated. (TIFF 201 kb)