10.6084/m9.figshare.c.3622250_D5.v1 Kyeong-Su Park Kyeong-Su Park Ju Kim Ju Kim Hee Shin Hee Shin Kyung-Sook Chung Kyung-Sook Chung Dong-Soo Im Dong-Soo Im Jung Lim Jung Lim Cho-Rok Jung Cho-Rok Jung Additional file 2: Figure S2. of E2-EPF UCP regulates stability and functions of missense mutant pVHL via ubiquitin mediated proteolysis Springer Nature 2015 VHL disease pVHL missense mutation E2-EPF UCP Ubiquitination Protein instability 2015-10-26 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_2_Figure_S2_of_E2-EPF_UCP_regulates_stability_and_functions_of_missense_mutant_pVHL_via_ubiquitin_mediated_proteolysis/4397393 Missense mutant pVHL constitute E3 ubiquitin complex in cell. (A) HEK293T cells were transfected with plasmid expressing GST tagged hotspot mutant pVHL and/or Flag tagged HIF-1Îą protein and then detected the interaction using GST-pull down assay. (B) HEK293T cells were transfected with plasmid expressing HA tagged mutant pVHL and/or GST tagged Elongin C protein and then detected the interaction using GST-pull down assay. Interacted mutants VHL proteins were detected by immunoblot as indicated. (TIFF 201 kb)