Additional file 5: Figure S5. of Overexpression of the miR-141/200c cluster promotes the migratory and invasive ability of triple-negative breast cancer cells through the activation of the FAK and PI3K/AKT signaling pathways by secreting VEGF-A Sul Choi Hoe Kim Tiefeng Jin Eun Hwang Minji Jung Woo Moon 10.6084/m9.figshare.c.3616277_D1.v1 https://springernature.figshare.com/articles/figure/Additional_file_5_Figure_S5_of_Overexpression_of_the_miR-141_200c_cluster_promotes_the_migratory_and_invasive_ability_of_triple-negative_breast_cancer_cells_through_the_activation_of_the_FAK_and_PI3K_AKT_signaling_pathways_by_secreting_VEGF-A/4379027 microRNA expression levels of miR-200 cluster transduced MCF-7, MDA-MB-231, HCC-38, and Hs578T cells. Quantitative real-time RT-PCR of microRNAs (miR-200a, miR-200b, and miR-200c). (A, B, C) microRNAs in MCF-7 cells. The miR-200ab cells transduced with the miR-200b/200a/429 cluster exhibited high levels of expression of miR-200a (~60-fold) and miR-200b (~40-fold) relative to those of the control cells. The miR-200c cells transduced with the miR-200c/141 cluster exhibited remarkably high levels of expression of miR-200c (~266-fold) relative to those of the control cells. (D, E, F) microRNAs in MDA-MB-231. The miR-200ab cells transduced with the miR-200b/200a/429 cluster exhibited high expression levels of miR-200a (~6-fold) and miR-200b (~40-fold) relative to those of the control cells. The miR-200c cells transduced with the miR-200c/141 cluster exhibited remarkably high expression levels of miR-200c (~93-fold) relative to those of the control cells. (G) microRNAs in HCC-38. The miR-200c cells transduced with the miR-200c/141 cluster exhibited remarkably high expression levels of miR-200c (~252-fold) relative to those of the control cells. (H) microRNAs in Hs578T. The miR-200c cells transduced with the miR-200c/141 cluster exhibited remarkably high expression levels of miR-200c (~205-fold) relative to those of the control cells. *p < 0.05, **p < 0.001. (JPG 50 kb) 2016-08-02 05:00:00 Triple-negative breast cancer (TNBC) microrna-200 (miR-200) Vascular endothelial growth factor (VEGF) Migration Invasion Phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) Protein kinase B (AKT) Focal adhesion kinase (FAK)