Additional file 1: Figure S1. of Pyrrolidine dithiocarbamate activates the Nrf2 pathway in astrocytes LiddellJeffrey LehtonenSarka DuncanClare Keksa-GoldsteineVelta LevonenAnna-Liisa GoldsteinsGundars MalmTarja WhiteAnthony KoistinahoJari KanninenKatja 2016 Effect of PDTC treatment on viability of astrocytes and hippocampal neurons. (A) Primary cultures of neonatal astrocytes were treated with increasing concentrations of PDTC for 24 h, after which cell viability was measured by LDH release and (B) MTT reduction assays. (C) Hippocampal neuron survival following 24-h PDTC treatment was measured by manual counting of viable cells/all cells following Hoechst staining. Data are expressed as mean % of viable cells ± SD and normalized to control cells in (B) and (C). **p < 0.001 relative to corresponding control.