Additional file 1: Figure S1. of 6-Mercaptopurine attenuates tumor necrosis factor-α production in microglia through Nur77-mediated transrepression and PI3K/Akt/mTOR signaling-mediated translational regulation Hsin-Yi Huang Hui-Fen Chang Ming-Jen Tsai Jhih-Si Chen Mei-Jen Wang 10.6084/m9.figshare.c.3613664_D2.v1 https://springernature.figshare.com/articles/figure/Additional_file_1_Figure_S1_of_6-Mercaptopurine_attenuates_tumor_necrosis_factor-_production_in_microglia_through_Nur77-mediated_transrepression_and_PI3K_Akt_mTOR_signaling-mediated_translational_regulation/4370960 LPS-induced activation of PKA and MSK1 are not suppressed by 6-MP. BV-2 cells were stimulated with 100 ng/ml LPS for the indicated times with or without 16 h pretreatment with 6-MP (50 μM). Immunoblots were set up to detect phosphorylated VASP (Ser157) and MSK1 (Ser376). Images are representative of three independent experiments. (TIF 400 kb) 2016-04-13 05:00:00 6-Mercaptopurine Microglia TNF-α Nur77 Nuclear factor-κB Histone H3 acetylation PI3K/Akt mTOR