Additional file 3: Figure S3. of BRG1 interacts with GLI2 and binds Mef2c gene in a hedgehog signalling dependent manner during in vitro cardiomyogenesis Joel Fair Anastassia Voronova Neven Bosiljcic Rashida Rajgara Alexandre Blais Ilona Skerjanc 10.6084/m9.figshare.c.3612392_D5.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_3_Figure_S3_of_BRG1_interacts_with_GLI2_and_binds_Mef2c_gene_in_a_hedgehog_signalling_dependent_manner_during_in_vitro_cardiomyogenesis/4366772 Modulation of HH signalling does not affect cell proliferation or survival. (A) Day 7 cells were treated with EdU for 1 h prior to staining. At least 5000 cells per sample, across 20 fields of view, were counted. Error bars represent +/- SEM. Student T-tests were used for statistical analyses. n = 3. (B) The yields of total RNA extracted from mES[Ctrl] and mES[GLI2] are given as a proxy for cell numbers in the cultures. For each time point, n is between 3 and 6, and error bars represent +/- SEM. (C) The proportion of cells with highly condensed, apoptotic nuclei after Hoechst staining at day 7 is given. At least 2500 cells per condition per replicate were counted. n = 2. (D) Yields of RNA in mES cultures treated with methanol vehicle or KAAD-cyclopamine. For each time point, n = 3, and error bars represent +/- SEM. (E) The proportion of cells with highly condensed, apoptotic nuclei is given for vehicle- and KAAD-cyclopamine-treated mES cultures at day 7. n = 2, and at least 500 cells per replicate and condition were counted. (PDF 415 kb) 2016-08-02 05:00:00 Cardiac development Cardiomyogenesis Hedgehog signalling pathway GLI2 BRG1