Additional file 3: Figure S2. of Assessment of global DNA methylation in the first trimester fetal tissues exposed to maternal cigarette smoking FaSvetlana LarsenTrine BildeKatrine DaugaardTina ErnstEmil OlesenRasmus MamsenLinn ErnstErik LarsenAgnete NielsenAnders 2016 DNA methylation in fetal livers. a DNA methylation in female and male fetal livers determined by the Elisa method using 5-mC-specific antibody (non-smoking female group N = 4, non-smoking male group N = 6, smoking female group N = 6, and smoking male group N = 5). Data represent the mean of three experiments. b Percentage of AluYb8 DNA methylation at 5 consecutive CpG sites (non-smoking group N = 15 and smoking group N = 18). c AluYb8 DNA methylation in males and females (non-smoking female group N = 6, non-smoking male group N = 9, smoking female group N = 8, and smoking male group N = 10). d Percentage of LINE-1 DNA methylation at three consecutive CpG sites (non-smoking group N = 15 and smoking group N = 18). e LINE-1 DNA methylation in males and females (non-smoking female group N = 6, non-smoking male group N = 9, smoking female group N = 8, and smoking male group N = 10). Percentages of methylation were calculated and displayed as described in the legend for Fig. 1. Unpaired t test was used to compare non-smoking and smoking exposed group, while two-way ANOVA was used to compare combined effects of smoke exposure and gender. *Indicates statistical significance, p < 0.05. (PDF 539 kb)