Graves, Hillary Wang, Pingping Lagarde, Matthew Chen, Zhihong Tyler, Jessica MOESM2 of Mutations that prevent or mimic persistent post-translational modifications of the histone H3 globular domain cause lethality and growth defects in Drosophila Additional file 2: Figure S2. H3S10p expression is not changed within cells that have mutations which prevent/mimic globular domain histone H3 modifications. A-N) Wing imaginal discs with GFP negative mutant clones generated using Ubx-FLP. Merged images show the nuclear marker DAPI in blue, H3S10p in magenta, and GFP+ and GFP- regions demarcate histone wild type cells and histone mutant cells, respectively. Grayscale images are the individual H3S10p channels. Within each mutant, we looked at GFP- clones within the zone of non-proliferation of the wing disc (see Sup. Fig. 4B) to determine if there was a consistent increase of H3S10p incorporation, which might indicate an increase of mitotic cells. Conversely, we looked at GFP- clones outside of the zone of non-proliferation to determine if there was a consistent decrease of H3S10p incorporation compared to their neighboring GFP+ control cells. Histone post-translational modifications;Drosophila;Imaginal discs;Histone H3 globular domain;Development 2016-02-29
    https://springernature.figshare.com/articles/presentation/MOESM2_of_Mutations_that_prevent_or_mimic_persistent_post-translational_modifications_of_the_histone_H3_globular_domain_cause_lethality_and_growth_defects_in_Drosophila/4360817
10.6084/m9.figshare.c.3610520_D3.v1