10.6084/m9.figshare.c.3609956_D5.v1 Thorsten Hartmann Thorsten Hartmann Sabrina Overhagen Sabrina Overhagen D. Ouwens D. Ouwens Silja Raschke Silja Raschke Paulus Wohlfart Paulus Wohlfart Norbert Tennagels Norbert Tennagels Nina Wronkowitz Nina Wronkowitz Jürgen Eckel Jürgen Eckel MOESM3 of Effect of the long-acting insulin analogues glargine and degludec on cardiomyocyte cell signalling and function Springer Nature 2016 Insulin glargine Insulin degludec Cardiac action Insulin analogues 2016-07-15 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/MOESM3_of_Effect_of_the_long-acting_insulin_analogues_glargine_and_degludec_on_cardiomyocyte_cell_signalling_and_function/4359236 Additional file 3: Figure S2. Phosphorylation of Akt(Thr308) in HL-1 cells. (A-C) HL-1 cells were used to assess the onset of insulin action by treatment with 200 nM for 5 (A); 10 (B) or 60 min (C) with insulin or insulin analogues. Phosphorylation of Akt(Thr308) was assessed by Western blot analysis. Data are normalised to tubulin levels. Representative blots are shown. Data represent mean values ± SEM, n = 3, *p < 0.05 vs. basal, #p < 0.05 vs. IDeg. Regular insulin (Ins), insulin glargine (IGla), active metabolite of insulin glargine (IGlaM1), insulin degludec (IDeg).