10.6084/m9.figshare.c.3604745_D5.v1
Hwanhee Kim
Hwanhee
Kim
Jun-Young Oh
Jun-Young
Oh
Sun-Lim Choi
Sun-Lim
Choi
Yeon-Ju Nam
Yeon-Ju
Nam
Anna Jo
Anna
Jo
Ara Kwon
Ara
Kwon
Eun-Young Shin
Eun-Young
Shin
Eung-Gook Kim
Eung-Gook
Kim
Hyong Kim
Hyong
Kim
Additional file 6: Figure S6. of Down-regulation of p21-activated serine/threonine kinase 1 is involved in loss of mesencephalic dopamine neurons
Springer Nature
2016
PAK1
Bcl-2
Apoptosis
Neurodegeneration
Parkinson’s disease
2016-04-27 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_6_Figure_S6_of_Down-regulation_of_p21-activated_serine_threonine_kinase_1_is_involved_in_loss_of_mesencephalic_dopamine_neurons/4343696
Other mutants of PAK1- inactive form reduce Bcl-2 level. a. HEK cells were transfected with 1, 2, or 3 μg of GFP, PAK1-WT, PAK1-H83,86L, or PAK1-K299R vectors and incubated for 48 h. The cell lysates were subjected to Western blotting analysis using anti-Myc, Bcl-2, Bcl-xL, or GAPDH antibody. b. The autoinhibitory domain (AID) of hPAK1 (67–150 amino acids) were amplified by PCR methods (hPAk1-67-R1-S: 5′-ggaattcggaagaaagagaaagagcggc-3′, hPAK1-150-Xho-A: 5′-ccgctcgagttaagctgacttatctgtaaagc-3′) and inserted to EcoRI/XhoI site of pCMV-Myc vector (Clontech) in-frame with Myc-tag, producing PAK1-DN (67–150) [47]. HEK cells were transfected with 1, 2, or 3 μg of PAK1-DN (67–150) vector and cell lysates were analyzed by Western blotting assay. (TIF 278 kb)