Additional file 10: Figure S5. of Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization Said Hafidh David Potěšil Jan Fíla Věra Čapková Zbyněk Zdráhal David Honys 10.6084/m9.figshare.c.3602213_D7.v1 https://springernature.figshare.com/articles/figure/Additional_file_10_Figure_S5_of_Quantitative_proteomics_of_the_tobacco_pollen_tube_secretome_identifies_novel_pollen_tube_guidance_proteins_important_for_fertilization/4336046 Prediction of transmembrane helices (TMHs) in pollen tube-secreted proteins and LocTree-associated GO terms. a Analysis of TMHs of pollen tube-secreted proteins using TMHMM algorithms. Right: validation of TMHMM algorithms using sequences from known Arabidopsis pollen tube plasma membrane proteins (ACA9, ANX1, ANX2, KRP2, KRP4) and non-TMH-containing pollen tube palmitoylated plasma membrane proteins LIP1 and LIP2 as controls. The absence of TMHs in pollen tube-secreted proteins also supports their potential secretion to the apoplast and extracellular matrix. b Independent prediction of pollen tube-secreted protein subcellular localization using the TargetP database. c Representative GO terms associated with the corresponding LocTree-predicted localization (Additional file 9: Figure S4) derived from PSI-Blast of pollen tube-secreted proteins. (TIF 780 kb) 2016-05-03 05:00:00 Protein secretion Pollen tube guidance Cell-cell signaling Double fertilization