Additional file 2: Figure S2. of The blockage of the Nogo/NgR signal pathway in microglia alleviates the formation of Aβ plaques and tau phosphorylation in APP/PS1 transgenic mice Yinquan Fang Lemeng Yao Chenhui Li Jing Wang Jianing Wang Shujian Chen Xin-fu Zhou Hong Liao 10.6084/m9.figshare.c.3598202_D2.v1 https://springernature.figshare.com/articles/journal_contribution/Additional_file_2_Figure_S2_of_The_blockage_of_the_Nogo_NgR_signal_pathway_in_microglia_alleviates_the_formation_of_A_plaques_and_tau_phosphorylation_in_APP_PS1_transgenic_mice/4324448 The levels of secreted Aβ40 and Aβ42 in BV-2 microglia-conditioned medium. Before added to the protein-coated wells, BV-2 microglia was transfected with Ctrl siRNA or NgR siRNA to suppress the expression of NgR. The cells were then exposed to PBS or Nogo-P4 for 6 h. And then, the conditioned medium was collected and centrifuged to discard cell debris. The release of Aβ40 (Figure A) and Aβ42 (Figure B) in the conditioned medium was determined using ELISA. Values were reported as mean ± SD. n = 3. (PDF 95 kb) 2016-03-03 05:00:00 Nogo NgR AD Microglia Neuron Tau Neuroinflammation