10.6084/m9.figshare.c.3597239_D7.v1 Ghulam Pir Ghulam Pir Bikash Choudhary Bikash Choudhary Eckhard Mandelkow Eckhard Mandelkow Eva-Maria Mandelkow Eva-Maria Mandelkow Additional file 11: of Tau mutant A152T, a risk factor for FTD/PSP, induces neuronal dysfunction and reduced lifespan independently of aggregation in a C. elegans Tauopathy model Springer Nature 2016 AD, Alzheimer disease PSP, Progressive supranuclear palsy FTD, Frontotemporal dementia CBD, Corticobasal degeneration 2016-04-27 05:00:00 Journal contribution https://springernature.figshare.com/articles/journal_contribution/Additional_file_11_of_Tau_mutant_A152T_a_risk_factor_for_FTD_PSP_induces_neuronal_dysfunction_and_reduced_lifespan_independently_of_aggregation_in_a_C_elegans_Tauopathy_model/4321472 Expression of mutant TauAT produces a substantial damage in GABAergic motor neurons. Representative whole worm maximum intensity projections (MIP) of the enlarged insets presented in main Fig. 2. Punc-25::gfp reporter that labels GABAergic inhibitory neurons with GFP, was crossed with respective tau-transgenic worms to visualize these neurons. GABAergic neurons show normal connectivity in non-tg worms, both dorsal and ventral nerve cords are intact (A). Expression of Tauwt produced dose dependent abnormalities in GABAergic neurons, with Tauwt -hi neurons (C) accumulating more damage than Tauwt -lo (B). However, mutant TauAT expression led to severe abnormalities in the form of gaps (arrowheads) in the dorsal and ventral nerve cords, in both TauAT-lo (D) and TauAT-hi (E). Also see Table 1 for detailed quantitative analysis. (PDF 276 kb)