Additional file 1 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens
Shantelle Claassen-Weitz
Sugnet Gardner-Lubbe
Kilaza S. Mwaikono
Elloise du Toit
Heather J. Zar
Mark P. Nicol
10.6084/m9.figshare.12292034.v1
https://springernature.figshare.com/articles/figure/Additional_file_1_of_Optimizing_16S_rRNA_gene_profile_analysis_from_low_biomass_nasopharyngeal_and_induced_sputum_specimens/12292034
Additional file 1. Four bacterial mock communities used to evaluate the effect of DNA extraction methods, storage buffers and bacterial biomass on 16S rRNA gene sequencing profiles. Zymobiomics-Primestore-high and Zymobiomics-Primestore-low: ZymoBIOMICS™ Microbial Community Standard bacterial cells in DNA/RNA Shield™ (Zymobiomics-Cells) suspended in PrimeStore® Molecular Transport medium (Primestore); Zymobiomics-STGG-high and Zymobiomics-STGG-low: Zymobiomics-Cells suspended in skim-milk tryptone glucose glycerol transport medium (STGG).
2020-05-13 03:40:23
16S rRNA gene
Bacteriome
Contamination
High-throughput sequencing
Low biomass
Mock controls
Negative controls
Optimization
Reproducibility
Respiratory