10.6084/m9.figshare.12292031.v1 Shantelle Claassen-Weitz Shantelle Claassen-Weitz Sugnet Gardner-Lubbe Sugnet Gardner-Lubbe Kilaza S. Mwaikono Kilaza S. Mwaikono Elloise du Toit Elloise du Toit Heather J. Zar Heather J. Zar Mark P. Nicol Mark P. Nicol Additional file 10 of Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens Springer Nature 2020 16S rRNA gene Bacteriome Contamination High-throughput sequencing Low biomass Mock controls Negative controls Optimization Reproducibility Respiratory 2020-05-13 03:40:22 Figure https://springernature.figshare.com/articles/figure/Additional_file_10_of_Optimizing_16S_rRNA_gene_profile_analysis_from_low_biomass_nasopharyngeal_and_induced_sputum_specimens/12292031 Additional file 10. Shifts in OTU-level proportions prior to and following the removal of “potential contaminants” using two in silico approaches for contaminant identification. Per specimen shifts (n = 148) in bacterial proportions are shown for eight OTUs classified as four genera A) Staphylococcus, B) Streptococcus, C) Acinetobacter and D) Stenotrophomonas. Open circles and smoothing splines (representing a factor of 2x the standard deviation) denote bacterial proportions (Y-axis) for each of the specimens (X-axis). Red: Proportions prior to decontamination; Blue: Proportions following the removal of “potential contaminants” identified using the “NTConly” approach; Yellow: Proportions following the removal of “potential contaminants” identified using the “NTC + decontam” approach.