%0 Journal Article %A Zhang, Lei %A Li, Yidong %A Wang, Qianchao %A Chen, Zhuo %A Li, Xiaoyun %A Wu, Zhuoxun %A Hu, Chaohua %A Liao, Dan %A Zhang, Wei %A Chen, Zhe-Sheng %D 2020 %T MOESM5 of The PI3K subunits, P110α and P110β are potential targets for overcoming P-gp and BCRP-mediated MDR in cancer %U https://springernature.figshare.com/articles/journal_contribution/MOESM5_of_The_PI3K_subunits_P110_and_P110_are_potential_targets_for_overcoming_P-gp_and_BCRP-mediated_MDR_in_cancer/11643660 %R 10.6084/m9.figshare.11643660.v1 %2 https://springernature.figshare.com/ndownloader/files/21126348 %K Cancer %K P-glycoprotein (P-gp/ABCB1/MDR1) %K Breast cancer resistance protein (BCRP/ABCG2/ABCP/MXR) %K Multidrug resistance (MDR) %K Reversal of MDR %K PI3K %K P110α/PIK3CA %K P110β/PIK3CB %X Additional file 5: Figure S5 JC-1 analysis of the apoptosis of KB-C2 and H460/MX80 cells and the derivative cells with deficiency of P110β. JC-1 staining indicating mitochondrial membrane potential showed severe depolarization of the mitochondrial membrane as a result of severe apoptosis of 110β subunit-k.o. KB-C2 cells (namely KB-C2-k.o. 110 β), as depicted by the green arrows. KB-C2 and H460/MX80 cells without apoptosis are depicted by arrows in slight blue. Slightly apoptotic 110β subunit-k.o. H460/MX80 (namely MX80-k.o.110β) cells are depicted by arrows in pink. The KB-C2 and H460/MX80 cells were seeded into 96-well plates (5 × 103 cells per well) and cultured for 8 h, followed by treatment with 1 μM of paclitaxel and 10 μM of mitoxantrone, respectively. JC-1 (2 μg mL− 1) was then used for cell staining for 30 min. The cells were washed with PBS and observed with fluorescent microscope. The experiments were independently repeated three times. %I figshare