MOESM2 of Carvacrol ameliorates acute campylobacteriosis in a clinical murine infection model Soraya Mousavi Anna-Maria Schmidt Ulrike Escher Sophie Kittler Corinna Kehrenberg Elisa Thunhorst Stefan Bereswill Markus Heimesaat 10.6084/m9.figshare.11557257.v1 https://springernature.figshare.com/articles/presentation/MOESM2_of_Carvacrol_ameliorates_acute_campylobacteriosis_in_a_clinical_murine_infection_model/11557257 Additional file 2: Figure S2. Representative photomicrographs illustrating apoptotic and proliferating epithelial as well as immune cells responses in large intestines upon carvacrol treatment of C. jejuni infected mice. Starting 4 days prior peroral C. jejuni infection on days 0 and 1, secondary abiotic IL-10−/− mice were treated with synthetic carvacrol (CARVA) or placebo (PLC) via the drinking water. Naive mice served as uninfected controls. Photomicrographs reepresentative for four independent experiments illustrate the average numbers of (A) apoptotic epithelial cells (Casp3+), (B) proliferating epithelial cells (Ki67+), (C) T lymphocytes (CD3+), and (D) B lymphocytes (B220+) in at least six high power fields (HPF) as quantitatively assessed in colonic paraffin sections applying in situ immunohistochemistry at day 6 post-infection (A: 400× magnification, scale bar 20 μm; B–D: 100× magnification, scale bar 100 μm). 2020-01-09 05:47:23 Carvacrol Anti-pathogenic and anti-inflammatory properties Campylobacter jejuni Secondary abiotic IL-10−/− mice Pro-inflammatory immune responses Bacterial translocation Host–pathogen-interaction Intestinal immunopathology Extra-intestinal and systemic immune responses