MOESM2 of Carvacrol ameliorates acute campylobacteriosis in a clinical murine infection model
Soraya Mousavi
Anna-Maria Schmidt
Ulrike Escher
Sophie Kittler
Corinna Kehrenberg
Elisa Thunhorst
Stefan Bereswill
Markus Heimesaat
10.6084/m9.figshare.11557257.v1
https://springernature.figshare.com/articles/presentation/MOESM2_of_Carvacrol_ameliorates_acute_campylobacteriosis_in_a_clinical_murine_infection_model/11557257
Additional file 2: Figure S2. Representative photomicrographs illustrating apoptotic and proliferating epithelial as well as immune cells responses in large intestines upon carvacrol treatment of C. jejuni infected mice. Starting 4 days prior peroral C. jejuni infection on days 0 and 1, secondary abiotic IL-10−/− mice were treated with synthetic carvacrol (CARVA) or placebo (PLC) via the drinking water. Naive mice served as uninfected controls. Photomicrographs reepresentative for four independent experiments illustrate the average numbers of (A) apoptotic epithelial cells (Casp3+), (B) proliferating epithelial cells (Ki67+), (C) T lymphocytes (CD3+), and (D) B lymphocytes (B220+) in at least six high power fields (HPF) as quantitatively assessed in colonic paraffin sections applying in situ immunohistochemistry at day 6 post-infection (A: 400× magnification, scale bar 20 μm; B–D: 100× magnification, scale bar 100 μm).
2020-01-09 05:47:23
Carvacrol
Anti-pathogenic and anti-inflammatory properties
Campylobacter jejuni
Secondary abiotic IL-10−/− mice
Pro-inflammatory immune responses
Bacterial translocation
Host–pathogen-interaction
Intestinal immunopathology
Extra-intestinal and systemic immune responses