%0 Journal Article %A Francescangeli, Federica %A Contavalli, Paola %A De Angelis, Maria Laura %A Careccia, Silvia %A Signore, Michele %A Haas, Tobias Longin %A Salaris, Federico %A Baiocchi, Marta %A Boe, Alessandra %A Giuliani, Alessandro %A Tcheremenskaia, Olga %A Pagliuca, Alfredo %A Guardiola, Ombretta %A Minchiotti, Gabriella %A Colace, Lidia %A Ciardi, Antonio %A D’Andrea, Vito %A La Torre, Filippo %A Medema, JanPaul %A De Maria, Ruggero %A Zeuner, Ann %D 2020 %T MOESM10 of A pre-existing population of ZEB2+ quiescent cells with stemness and mesenchymal features dictate chemoresistance in colorectal cancer %U https://springernature.figshare.com/articles/journal_contribution/MOESM10_of_A_pre-existing_population_of_ZEB2_quiescent_cells_with_stemness_and_mesenchymal_features_dictate_chemoresistance_in_colorectal_cancer/11543352 %R 10.6084/m9.figshare.11543352.v1 %2 https://springernature.figshare.com/ndownloader/files/20734623 %K Colorectal cancer %K Chemotherapy resistance %K Dormancy %K Quiescence %K Epithelial-to-mesenchymal transition %K Cancer stem cells %X Additional file 10: Figure S4. In vivo effects of ZEB2 overexpression in CCSCs. a Volume of xenografts derived from CCSCs transduced with pLenti-GFP (Vector, black line/triangles) or with pLenti-GFP-ZEB2 (ZEB2, red line/squares). Graph shows the mean ± SEM, 6 tumors/group. **P < 0.01 from two-tailed t test. b Left panels: representative confocal images of xenograft sections derived from tumors obtained with primary cells transduced with pLenti-GFP (Vector) and GFP-ZEB2 (ZEB2). Sections were stained with anti-Ki67 (red). Scale bar, 70 μm. Quantification (right panel) was performed on 5 fields /group. *P < 0.05 from two-tailed t test. AU, arbitrary units. c Cell cycle analysis of GFP+ cells FACS-isolated from Vector and ZEB2-transduced tumors obtained with primary cells. d qRT-PCR analysis of the indicated transcripts in Vector- and ZEB2-transduced tumors obtained with primary cells, n = 3. *P < 0.05 and **P < 0.01 from two-tailed t test. n.s. = not significant by t test. Values are the mean ± SD. %I figshare