MOESM7 of DUF3669, a “domain of unknown function” within ZNF746 and ZNF777, oligomerizes and contributes to transcriptional repression
Mohannad Al Chiblak
Felix Steinbeck
Hans-Jürgen Thiesen
Peter Lorenz
10.6084/m9.figshare.11416248.v1
https://springernature.figshare.com/articles/journal_contribution/MOESM7_of_DUF3669_a_domain_of_unknown_function_within_ZNF746_and_ZNF777_oligomerizes_and_contributes_to_transcriptional_repression/11416248
Additional file 7. Analysis of stable HEK293 cell lines expressing GST alone or GST-Z746a/1–279 Western blot analysis of total protein extracts from six clones that survived under the selection of hygromycin B (2 express GST and 4 express GST-Z746a/1–279) alongside extracts from parent cell line Flp-in T-REx-239 cells (negative control) and transiently transfected parent cells expressing GST or GST-Z746a/1–279 (positive controls). Extracts made with 1x SDS sample buffer after a 24-h induction of expression with 2 μg/ml tetracycline. The blot was probed with anti-GST (depicted in the upper part) and anti-GAPDH (lower part) antibodies. Protein bands of the expected size are indicated by arrows. * indicates unspecific bands recognized by the antibodies.
2019-12-20 06:34:50
ZNF746
ZNF777
KRAB
ZNF10/KOX1
DUF3669
TRIM28
Parkinson’s disease
HAP1
Transcription
Repression