MOESM4 of Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects Guoxing Zheng Qingqing Zhu Junchao Dong Xin Lin Chengming Zhu 10.6084/m9.figshare.10279070.v1 https://springernature.figshare.com/articles/figure/MOESM4_of_Rapid_generation_and_selection_of_Cas9-engineering_TRP53_R172P_mice_that_do_not_have_off-target_effects/10279070 Additional file 4: Figure S4. p53 level in the MEFs upon the stimulation of UV radiation. The protein levels of p53 in MEFs of various genotypes are compared upon UV stimulation of indicated time. The result showed that the expression of p53 increased in all Homozygous MEF cells. β-Actin worked as normalization control. 2019-11-09 05:10:49 Human genetic diseases Mouse model TRP53-R172P mutation CRISPR/Cas9 system Off-target effects