10.6084/m9.figshare.10041503.v1 Liankang Sun Liankang Sun Yufeng Wang Yufeng Wang Liang Wang Liang Wang Bowen Yao Bowen Yao Tianxiang Chen Tianxiang Chen Qing Li Qing Li Zhikui Liu Zhikui Liu Runkun Liu Runkun Liu Yongshen Niu Yongshen Niu Tao Song Tao Song Qingguang Liu Qingguang Liu Kangsheng Tu Kangsheng Tu Additional file 3: of Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP Springer Nature 2019 Hepatocellular carcinoma Cancer-associated fibroblasts COMP Resolvin D1 Cancer stemness ROS FOXM1 2019-10-24 13:52:46 Figure https://springernature.figshare.com/articles/figure/Additional_file_3_of_Resolvin_D1_prevents_epithelial-mesenchymal_transition_and_reduces_the_stemness_features_of_hepatocellular_carcinoma_by_inhibiting_paracrine_of_cancer-associated_fibroblast-derived_COMP/10041503 Figure S1. Identification of CAFs derived from HCC patients. (A) CAFs and PTFs were isolated from human HCC tissues and adjacent normal liver tissues. α-SMA expression in CAFs and PTFs were determined by immunofluorescence staining. The magnification of the picture is 400×. Scale bars = 20 μm. (B) qRT-PCR was performed to detect the expression of COMP and α-SMA at mRNA level. n = three independent experiments, **P < 0.01 versus control by t test. (C) Expression levels of Col 1α, fibronectin and α-SMA in CAFs and PTFs were determined by western blotting. * P < 0.05 or ** P < 0.01versus control by t test. (TIF 806 kb)