MOESM3 of Dynamic response of microglia/macrophage polarization following demyelination in mice ChuTianci ZhangYi TianZhisen YeChuyuan ZhuMingming ShieldsLisa KongMaiying BarnesGregory ShieldsChristopher CaiJun 2019 Additional file 3: Figure S1. Temporal characteristics of glial cell proliferation following dorsal demyelination. (A-C) Representative photomicrographs of proliferative (A) OPCs (Pdgfrα+Ki67+), (B) M/M (Iba1+Ki67+), and (C) astrocytes (GFAP+Ki67+) in the dorsal demyelination area in the CTR, LPC, and LPS groups at 3, 10, and 21 dpi. Dashed white line indicates the margins of the dorsal columns. Representative double labelling is indicated by arrowhead and single labelling is indicated by arrow in the inset for better view. Scale bar, 50 μm for panel figure; 20 μm for inset. (D-F) Quantification of the cell density of proliferative (D) OPCs (Pdgfrα+Ki67+ cells/mm2), (E) M/M (Iba1+Ki67+ cells/mm2), and (F) astrocytes (GFAP+Ki67+ cells/mm2) within the dorsal column lesion site in the three groups at 3, 10, and 21 dpi. Cell densities was compared to the CTR group (** p < 0.01, *** p < 0.001, **** p < 0.0001) and the LPC group (× p < 0.05, × × × p < 0.001, × × × × p < 0.0001) at each given time-point; for each group, cell density was compared to 3 dpi (## p < 0.01, ### p < 0.001, #### p < 0.0001) and 10 dpi (+++ p < 0.001, ++++ p < 0.0001). (G, H) Proportions of proliferative OPCs (Pdgfrα+Ki67+), M/M (Iba1+Ki67+), and astrocytes (GFAP+Ki67+) in total proliferative cells (Ki67+) within the dorsal column at the lesion site in (G) LPC and (H) LPS groups at 3, 10, and 21 dpi. The cell proportion was compared to 3 dpi (# p < 0.05, ## p < 0.01, #### p < 0.0001) and 10 dpi (++ p < 0.01, ++++ p < 0.0001) for each group. Data were collected from three animals per group at each time-point (n = 3 mice).